Acetate Differential Agar
For the differentiation of Shigella species from those of the Escherichia genus.
Application
For the differentiation of Shigella species from those of the Escherichia genus.
Specifications
| PHYSICAL PARAMETERS |
|
| Description |
A fine, free flowing, hygroscopic powder |
| Color |
Greenish beige colored powder |
| Solubility |
Soluble in Distilled / de-ionized water at 50˚C |
| Color |
Forest green colored slightly opalescent |
| pH |
6.7±0.2 at 25 ˚C (29.18 g/ l) |
| Solidification |
40˚C |
| Color |
Forest green colored slightly opalescent gel |
Contents
| Ingredients |
Gram/Litre |
| Sodium acetate |
2.000 |
| Magnesium sulphate |
0.100 |
| Sodium chloride |
5.000 |
| Monoammonium phosphate |
1.000 |
| Dipotassium hydrogen phosphate |
1.000 |
| Bromothymol Blue |
0.080 |
| Agar |
20.000 |
| pH at 25˚C |
6.7±0.2 |
Directions
Dissolve 29.18 grams in 1000 ml distilled water. Boil to dissolve the medium completely and sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 min, cool it to 42-45 °C and distribute aseptically in test tubes. Slant the tubes to have a small butt and slant, allow to solidify. Ensure complete solidification and inoculate test sample aseptically.
