Actinomyces Broth
For the maintenance and cultivation of Actinomyces species
Application
For the maintenance and cultivation of Actinomyces species
Specifications
| PHYSICAL PARAMETERS |
|
| Description |
A fine, free flowing, hygroscopic powder |
| Color |
Beige colored powder. |
| Solubility |
Soluble in Distilled / de-ionized water at 50˚C |
| Color |
Light amber colored slightly opalescent solution with a slight precipitate. |
| pH |
7.2 ±0.2 at 25˚C (57.22 g/ l) |
Contents
| Ingredients |
Gram/Litre |
| Beef Heart Infusion |
10.000 |
| Tryptose |
10.000 |
| Casein Enzymic Hydrolysate |
4.000 |
| Yeast Extract |
5.000 |
| Dextrose |
5.000 |
| L-Cysteine Hydrochloride |
1.000 |
| Starch Soluble |
1.000 |
| Sodium Chloride |
5.000 |
| Monopotassium Phosphate |
15.000 |
| Ammonium Sulphate |
1.000 |
| Magnesium Sulphate |
0.200 |
| Calcium Chloride |
0.020 |
| pH at 25⁰C |
7.2 ±0.2 |
Directions
Dissolve 57.22 grams in 1000 ml distilled water, boil to dissolve the medium
completely and sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 min, cool it to 42-45 °C
and inoculate test sample aseptically.
