Actinomycete Isolation Agar
For isolation and propagation of Actinomycetes from soil and water.
Application
For isolation and propagation of Actinomycetes from soil and water.
Specifications
| PHYSICAL PARAMETERS |
|
| Description |
A fine, free flowing, hygroscopic powder. |
| Color |
Light beige colored powder. |
| Solubility |
Soluble in Distilled/de-ionized water at 50°C |
| Color |
Light amber colored opalescent solution |
| pH |
8.1 ±0.2 at 25℃ (22.00 g/ l) |
| Solidification |
40 ˚C |
| Color |
Light amber colored opalescent gel |
Contents
| Ingredients |
Gram/Litre |
| Sodium Caseinate |
2.000 |
| L-Asparagine |
0.100 |
| Sodium Propionate |
4.000 |
| Dipotassium Phosphate |
0.500 |
| Magnesium Sulphate |
0.100 |
| Ferrous Sulphate |
0.001 |
| Agar |
15.000 |
| pH at 25 ˚C |
8.1 ±0.2 |
Directions
Dissolve 22.00 grams in 5% glycerol solution, boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 min, cool it to 42-45 °C and distribute aseptically in petri plates. Ensure complete solidification and inoculate test sample aseptically.
