Anaerobic Agar
For cultivation of anaerobic bacteria especially Clostridium species.
Application
For cultivation of anaerobic bacteria especially Clostridium species.
Specifications
| PHYSICAL PARAMETERS |
|
| Description |
A fine, free flowing, hygroscopic powder |
| Color |
Light beige colored powder. |
| Solubility |
Soluble in Distilled/de-ionized water at 50°C |
| Color |
Light amber colored opalescent medium |
| pH |
7.2 ±0.2 at 25 ˚ C (58.00 g/ l) |
| Solidification |
40 ˚C |
| Color |
Light green color red slightly opalescent gel |
Contents
| Ingredients |
Gram/Litre |
| Tryptone |
20.000 |
| Dextrose |
10.000 |
| Sodium chloride |
5.000 |
| Sodium thioglycolate |
2.000 |
| Sodium formaldehyde sulfoxylate |
1.000 |
| Methylene blue |
0.002 |
| Agar |
20.000 |
| pH at 25˚C |
7.2 ±0.2 |
Directions
Dissolve 58.00 grams in 1000 ml distilled water. Boil to dissolve the medium completely and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 min, cool it to 42-45 °C and distribute aseptically in test tubes or petri plates. Allow to solidify and inoculate test sample aseptically.
