Anaerobic Thioglycollate Medium Base
For the cultivation of anaerobes.
Application
For the cultivation of anaerobes.
Specifications
| PHYSICAL PARAMETERS |
|
| Description |
A fine, free flowing, hygroscopic powder |
| Color |
Beige colored powder. |
| Solubility |
Soluble in Distilled/de-ionized water at 50°C |
| Color |
Medium to dark amber color, clear to slightly opalescent gel |
| pH |
7.3±0.2 at 25℃ (40.55 g/ l) |
Contents
| Ingredients |
Gram/Litre |
| Casein enzymic hydrolysate |
17.000 |
| Papaic digest of soyabean meal |
3.000 |
| Meat extract |
7.500 |
| Liver hydrolysate |
3.000 |
| Dextrose |
6.000 |
| Sodium chloride |
2.500 |
| Sodium thioglycollate |
0.500 |
| L-Cysteine |
0.250 |
| Sodium sulphite |
0.100 |
| Agar |
0.700 |
| pH at 25˚C |
7.3±0.2 |
Directions
Dissolve 40.55 grams in 900 ml distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 min, cool it to 42-45°C. Add 100 ml sterile serum and mix well. Dispense into a sterile test tube and inoculate the test sample aseptically.
