Andrade Peptone Water
As a basal medium to study fermentation reactions by adding carbohydrates.
Application
As a basal medium to study fermentation reactions by adding carbohydrates.
Specifications
| PHYSICAL PARAMETERS |
|
| Description |
A fine, free flowing, hygroscopic powder |
| Color |
Pinkish beige colored powder. |
| Solubility |
Soluble in Distilled/de-ionized water at 50°C |
| Color |
Light pink colored clear solution without any precipitate. |
| pH |
7.4 ±0.2 at 25˚C (15.00 g/ l) |
Contents
| Ingredients |
Gram/Litre |
| Peptone |
10.000 |
| Sodium Chloride |
5.000 |
| Andrade Indicator |
0.100 |
| pH at 25˚C |
7.4±0.2 |
Directions
Dissolve 15.10 grams in 1000 ml distilled water. Boil to dissolve the medium completely and distribute aseptically in test tubes containing inverted Durham’s tubes. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to room temperature and aseptically add sterile stock solution of carbohydrate to a final concentration of 0.5% to 1.0% (w/v).
