Antibiotic Assay Medium A
For microbiological diffusion assay of several antibiotics in accordance with EP.
Application
For microbiological diffusion assay of several antibiotics in accordance with EP.
Specifications
| PHYSICAL PARAMETERS |
|
| Description |
A fine, free flowing, hygroscopic powder |
| Color |
Beige colored powder. |
| Solubility |
Soluble in Distilled/de-ionized water at 50°C |
| Color |
Light amber colored opalescent |
| pH |
6.55 ±0.05 at 25℃ (30.50 g/ l) |
| Solidification |
40 ˚C |
| Color |
Light amber colored opalescent gel |
Contents
| Ingredients |
Gram/Litre |
| Peptone |
6.000 |
| Tryptone |
4.000 |
| Yeast Extract |
3.000 |
| Meat Extract |
1.500 |
| Dextrose |
1.000 |
| Agar |
15.000 |
| pH at 25˚C |
6.55 ± 0.05 |
Directions
Dissolve 30.50 grams in 1000 ml distilled water. Boil to dissolve the medium completely and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 min, cool it to 42-45 °C and distribute aseptically in petri plates. Ensure complete solidification and inoculate test sample aseptically.
