Antibiotic Assay Medium No.2 (Base Agar)
For microbiological assay of antibiotics.
Application
For microbiological assay of antibiotics.
Specifications
| PHYSICAL PARAMETERS |
|
| Description |
A fine, free flowing, hygroscopic powder |
| Color |
Beige colored powder. |
| Solubility |
Soluble in Distilled/de-ionized water at 50°C |
| Color |
Light amber colored opalescent |
| pH |
6.6 ±0.2 at 25 ˚C (25.50 g/ l) |
| Solidification |
40 ˚C |
| Color |
Light amber colored opalescent gel |
Contents
| Ingredients |
Gram/Litre |
| Peptone |
6.000 |
| Meat extract |
1.500 |
| Yeast Extract |
3.000 |
| Agar |
15.000 |
| pH at 25˚C |
6.6 ±0.2 |
Directions
Dissolve 25.50 grams in 1000 ml distilled water. Boil to dissolve the medium completely and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 min, cool it to 42-45 °C and distribute aseptically in petri plates. Ensure complete solidification and aseptically add Antibiotic assay medium No. 1 as seed agar, inoculated with the microbial culture.
