Antibiotic Assay Medium No.3 (Assay Broth)
For microbiological assay of antibiotics.
Application
For microbiological assay of antibiotics.
Specifications
| PHYSICAL PARAMETERS |
|
| Description |
A fine, free flowing, hygroscopic powder. |
| Color |
Beige colored powder. |
| Solubility |
Soluble in Distilled/de-ionized water at 50°C |
| Color |
Light amber colored opalescent |
| pH |
7.0 ±0.05 at 25 ˚C (17.50 g/ l) |
Contents
| Ingredients |
Gram/Litre |
| Peptone |
5.000 |
| Yeast Extract |
1.500 |
| Meat Extract |
1.500 |
| Sodium Chloride |
3.500 |
| Dextrose |
1.000 |
| Dibasic Potassium Phosphate |
3.680 |
| Monobasic Potassium Phosphate |
1.320 |
| pH after sterilization at 25˚C |
7.0 ±0.05 |
Directions
Dissolve 17.50 grams in 1000 ml distilled water. Boil to dissolve the medium completely and distribute in test tubes. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 min, cool it to 42-45 °C and add desired amount of antibiotic solution in it and inoculate uniform volume of bacterial suspension in series of tubes.
