Antibiotic Assay Medium No. 10 (Polymyxin Seed Agar)
As a seed layer medium for assaying the products containing Polymyxin-B, Carbenicillin,
Colistin & Colistimethate sodium.
Application
As a seed layer medium for assaying the products containing Polymyxin-B, Carbenicillin,
Colistin & Colistimethate sodium.
Specifications
| PHYSICAL PARAMETERS |
|
| Description |
A fine, free flowing, hygroscopic powder |
| Color |
Beige colored powder. |
| Solubility |
Soluble in Distilled / de-ionized water at 50˚C |
| Color |
Light amber colored opalescent |
| pH |
7.2 ±0.2 at 25˚C (42.00 g/ l) (10 ml/l polysorbate 80) |
| Solidification |
40˚C |
| Color |
Light amber colored opalescent gel |
Contents
| Ingredients |
Gram/Litre |
| Pancreatic Digest of Casein |
17.000 |
| Papaic Digest of Soybean |
3.000 |
| Sodium Chloride |
5.000 |
| Dibasic Potassium Phosphate |
2.500 |
| Dextrose |
2.500 |
| Agar |
12.000 |
| pH at 25˚C |
7.2 ±0.2 |
Directions
Dissolve 42.00 grams in 1000 ml distilled water. Boil to dissolve the medium
completely add 10 ml of Polysorbate 80. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15
min, cool it to 42-45 °C and distribute aseptically in petri plates. Ensure complete solidification
and inoculate test sample aseptically.
