Antibiotic Assay Medium No.32
For microbiological assay of Dihydrostreptomycin and Vancomycin by preparing inoculum
of Bacillus subtilis ATCC 6633
Application
For microbiological assay of Dihydrostreptomycin and Vancomycin by preparing inoculum
of Bacillus subtilis ATCC 6633
Specifications
| PHYSICAL PARAMETERS |
|
| Description |
A fine, free flowing, hygroscopic powder |
| Color |
Light beige colored powder. |
| Solubility |
Soluble in Distilled / de-ionized water at 50˚C |
| Color |
Light amber colored opalescent |
| pH |
6.6 ±0.1 at 25˚C (30.80 g/ l) |
| Solidification |
40˚C |
| Color |
Light amber colored opalescent gel |
Contents
| Ingredients |
Gram/Litre |
| Peptone |
6.000 |
| Casein peptone |
4.000 |
| Yeast Extract |
3.000 |
| Meat Extract |
1.500 |
| Dextrose |
1.000 |
| Manganese sulphate |
0.300 |
| Agar |
15.000 |
| pH at 25˚C (after sterilization) |
6.6 ±0.1 |
Directions
Dissolve 30.80 grams in 1000 ml distilled water. Boil to dissolve the medium
completely and sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 min, cool it to 42-45 °C,
and distribute aseptically in sterile petri plates. Ensure complete solidification and inoculate test
organisms aseptically.
