Ashby’s Glucose Agar
For the cultivation of Azotobacter species that can utilize mannitol as carbon and
atmospheric nitrogen as nitrogen source.
Application
For the cultivation of Azotobacter species that can utilize mannitol as carbon and
atmospheric nitrogen as nitrogen source.
Specifications
| PHYSICAL PARAMETERS |
|
| Description |
A fine, free flowing, hygroscopic powder |
| Color |
Off white colored powder |
| Solubility |
Soluble in Distilled / de-ionized water at 50˚C |
| Color |
Milky white colored solution with stealing at bottom on standing |
| pH |
7.4 ±0.2 at 25℃ (40.70 g/ l) |
| Solidification |
40˚C |
| Color |
Milky white colored opalescent gel with slight precipitate |
Contents
| Ingredients |
Gram/Litre |
| Glucose |
20.00 |
| Dipotassium Phosphate |
0.20 |
| Magnesium Sulphate |
0.20 |
| Sodium Chloride |
0.20 |
| Potassium Sulphate |
0.10 |
| Calcium Carbonate |
5.00 |
| Agar |
15.00 |
| pH at 25˚C |
7.4 ±0.2 |
Directions
Dissolve 40.70 grams in 1000 ml distilled water. Sterilize by autoclaving at 15 lbs
pressure (121 °C) for 15 min, cool it to 42-45 °C and distribute in desire. Ensure complete
solidification and inoculate test sample aseptically.
