Ashby’s Mannitol Agar
For isolation of Azotobacter species from soil that can use mannitol and atmospheric
nitrogen as source of carbon and nitrogen respectively.
Application
For isolation of Azotobacter species from soil that can use mannitol and atmospheric
nitrogen as source of carbon and nitrogen respectively.
Specifications
| PHYSICAL PARAMETERS |
|
| Description |
A fine, free flowing, hygroscopic powder |
| Color |
Off white colored powder |
| Solubility |
Soluble in Distilled / de-ionized water at 50˚C |
| Color |
Milky white colored solution with stealing at bottom on standing |
| pH |
7.4 ±0.2 at 25℃ (40.70 g/ l) |
| Solidification |
40˚C |
| Color |
Milky white colored opalescent gel with slight precipitate |
Contents
| Ingredients |
Gram/Litre |
| Mannitol |
20.00 |
| Dipotassium Phosphate |
0.20 |
| Magnesium Sulphate |
0.20 |
| Sodium Chloride |
0.20 |
| Potassium Sulphate |
0.10 |
| Calcium Carbonate |
5.00 |
| Agar |
15.00 |
| pH at 25˚C |
7.4 ±0.2 |
Directions
Dissolve 40.70 grams in 1000 ml distilled water. Sterilize by autoclaving at 15 lbs
pressure (121 °C) for 15 min, cool it to 42-45 °C and distribute in desire. Ensure complete
solidification and inoculate test sample aseptically.
