Ashby’s Mannitol Broth
For isolation of Azotobacter species from soil.
Application
For isolation of Azotobacter species from soil.
Specifications
| PHYSICAL PARAMETERS |
|
| Description |
A fine, free flowing, hygroscopic powder |
| Color |
Off white colored powder. |
| Solubility |
Soluble in Distilled / de-ionized water at 50˚C |
| Color |
Milky white colored solution with stealing at bottom on standing. |
| pH |
7.4 ±0.2 at 25℃ (25.70 g/ l) |
Contents
| Ingredients |
Gram/Litre |
| Mannitol |
20.00 |
| Dipotassium Phosphate |
0.20 |
| Magnesium Sulphate |
0.20 |
| Sodium Chloride |
0.20 |
| Potassium Sulphate |
0.10 |
| Calcium Carbonate |
5.00 |
| pH at 25˚C |
7.4 ±0.2 |
Directions
Dissolve 25.70 grams in 1000 ml distilled water. Sterilize by autoclaving at 15 lbs
pressure (121 °C) for 15 min, cool it to 42-45 °C and inoculate test sample aseptically.
