Asparagine Proline Broth
For the cultivation of Pseudomonas aeruginosa by membrane filter technique.
Application
For the cultivation of Pseudomonas aeruginosa by membrane filter technique.
Specifications
| PHYSICAL PARAMETERS |
|
| Description |
A fine, free flowing, hygroscopic powder |
| Color |
Off white colored powder. |
| Solubility |
Soluble in Distilled / de-ionized water at 50˚C |
| Color |
Colorless clear solution, without any precipitate |
| pH |
7 .2 ±0.2 at 25℃ (14.50 g/ l) |
Contents
| Ingredients |
Gram/Litre |
| DL-Asparagine |
2.00 |
| L-Proline |
1.00 |
| Dipotassium phosphate, anhydrous |
1.00 |
| Magnesium Sulphate |
0.50 |
| Potassium Sulphate |
10.00 |
| pH at 25˚C |
7.2 ±0.2 |
Directions
Dissolve 14.50 grams in 900 ml distilled water, boil to dissolve the medium
completely allow to cool at room temperature and add 25ml of ethanol and dilute up to 1000 ml
with distilled water. Distribute as desired in screw-capped bottles. Do not used polypropylene
caps with seal. Close the caps so that the seal in the lid just touches the lip of the bottle. Sterilize
by autoclaving at 15 lbs pressure (121 °C) for 15 min, cool it to 42-45 °C and inoculate test
sample aseptically. Alternatively, sterilize the ethanol by filtration through the cellulose acetate
or nitrate membrane of 0.22 µm and then aseptically to the medium after autoclaving and
cooling.
