Aspergillus Differentiation Medium Base
For detection of aflatoxin producing Aspergillus species from food samples.
Application
For detection of aflatoxin producing Aspergillus species from food samples.
Specifications
| PHYSICAL PARAMETERS |
|
| Description |
A fine, free flowing, hygroscopic powder |
| Color |
Beige colored powder. |
| Solubility |
Soluble in Distilled / de-ionized water at 50˚C |
| Color |
Light amber, slightly opalescent |
| pH |
6.3 ±0.2 at 25 ˚C (45.50 g/ l) |
| Solidification |
40˚C |
| Color |
Light amber color opalescent gel |
Contents
| Ingredients |
Gram/Litre |
| Peptone |
10.000 |
| Yeast Extract |
20.000 |
| Ferric ammonium citrate |
0.500 |
| Dichloran |
0.002 |
| Agar |
15.000 |
| pH at 25˚C |
6.3 ±0.2 |
Directions
Dissolve 45.50 grams in 1000 ml distilled water. Boil to dissolve the medium
completely and sterilize by autoclaving at 15 lbs. pressure (121 °C) for 15 min, cool it to 42-45 °C,
aseptically add Chloramphenicol Selective Supplement (100 mg). Mix well and pour into sterile
petri plates. Avoiding air bubbles while pouring. Ensure complete solidification and inoculate
test sample aseptically.
