Azide Dextrose Broth
For detection of faecal Streptococci in water, sewage, food and other materials.
Application
For detection of faecal Streptococci in water, sewage, food and other materials.
Specifications
| PHYSICAL PARAMETERS |
|
| Description |
A fine, free flowing, hygroscopic powder |
| Color |
Beige colored powder. |
| Solubility |
Soluble in Distilled / de-ionized water at 50˚C |
| Color |
Light amber colored clear solution |
| pH |
7.2 ±0.2 at 25 ˚C (34.7.00 g/ l) |
Contents
| Ingredients |
Gram/Litre |
| Tryptose |
15.00 |
| Meat extract |
4.50 |
| Dextrose |
7.50 |
| Sodium chloride |
7.50 |
| Sodium azide |
0.20 |
| pH at 25˚C |
7.2 ±0.2 |
Directions
Dissolve 34.70 grams in 1000 ml distilled water. Boil to dissolve the medium
completely and distribute aseptically. Sterilize by autoclaving at 15 lbs pressure (121 °C) for 15
min, cool it to 42-45 °C and inoculate test sample aseptically. For double strength media
preparation dissolve twice amount (69.40 g/l) of dehydrated media in 1000 ml distilled water.
