Azospirillum Medium w/ Agar (Twin Pack)
For the cultivation of Azospirillum species.
Application
For the cultivation of Azospirillum species.
Specifications
| PHYSICAL PARAMETERS |
|
| Description |
A fine, free flowing, hygroscopic powder |
| Color |
Part A: Cream to yellow homogeneous free flowing powder Part B: White to cream pellets. |
| Solubility |
Soluble in Distilled / de-ionized water at 50˚C |
| Color |
Light yellow to pale green colored solution |
| pH |
6.8 ±0.2 at 25℃ (8.10g part A and 4g part B) |
Contents
| Ingredients |
Gram/Litre |
| Part A |
|
| Malic acid |
5.000 |
| Dipotassium hydrogen phosphate |
0.500 |
| Ferrous Sulphate |
0.500 |
| Manganese Sulphate |
0.010 |
| Magnesium Sulphate |
0.200 |
| Sodium Chloride |
0.100 |
| Bromothymol blue |
0.002 |
| Sodium molybdate |
0.002 |
| Calcium chloride |
0.020 |
| Agar |
1.750 |
| Part B |
|
| Potassium hydroxide |
4.000 |
| pH at 25˚C |
6.8 ±0.2 |
Directions
Part B: Dissolve 4 grams of part B in 50 ml of sterile distilled water. Part A:
Dissolve 8.10 grams in 950 ml distilled water, boil to dissolve the medium completely. Sterilize by
autoclaving at 15 lbs pressure (121 °C) for 15 min, cool it to 42-45 °C. Mix 950 ml of part A with
50 ml of part B in 1 liter sterile container, mix well and dispense desired.
