Azotobacter Agar (Mannitol)
For isolation, cultivation and identification of Mannitol positive Azotobacter species from
soil.
Application
For isolation, cultivation and identification of Mannitol positive Azotobacter species from
soil.
Specifications
| PHYSICAL PARAMETERS |
|
| Description |
A fine, free flowing, hygroscopic powder |
| Color |
Light beige colored powder. |
| Solubility |
Soluble in Distilled / de-ionized water at 50˚C |
| Color |
Clear slightly opalescent solution |
| pH |
7.6 ±0.2 at 25 ˚C (25.40 g/ l) |
| Solidification |
40˚C |
| Color |
Clear slightly opalescent gel |
Contents
| Ingredients |
Gram/Litre |
| Dipotassium Hydrogen Phosphate |
0.050 |
| Potassium Hydrogen Phosphate |
0.150 |
| Magnesium Sulphate |
0.200 |
| Calcium Chloride |
0.020 |
| Sodium Ammonium Molybdate |
0.002 |
| Ferric Chloride |
0.00009 |
| Mannitol |
10.000 |
| Agar |
15.000 |
| pH at 25˚C |
7.6 ± 0.2 |
Directions
Dissolve 25.40 grams in 1000 ml distilled water, boil to dissolve the medium
completely. Sterilize by autoclaving at 15 lbs. pressure (121 °C) for 15 min, cool it to 42-45 °C
and distribute aseptically in sterile petri plates, allow to solidify. Ensure complete solidification
and inoculate test sample aseptically.
