Dissolve 24.00 grams in 1000 ml distilled water. Boil to dissolve the medium completely and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 min, cool it to 42-45 °C and distribute aseptically in petri plates. Ensure complete solidification and inoculate test sample aseptically. Tryptone Glucose Yeast Extract Agar
For enumeration of bacteria in water, air, milk and dairy products.